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Western blot analysis in HepG2 cell line lysates (35ug/lane).
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in HepG2 cell line lysates (35ug/lane).

SEC13 Antibody

CATALOG NUMBER: 55-380

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This SEC13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 72-100 amino acids from the Central region of human SEC13.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
36 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SEC13
Additional Names:
Protein SEC13 homolog, SEC13-like protein 1, SEC13-related protein, SEC13, D3S1231E, SEC13L1, SEC13R
Protein Accession Number:
P55735
Protein Gi Number:
50403748
Ncbi Gene Id Number:
6396
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene belongs to the SEC13family of WD-repeat proteins. It is a constituent of theendoplasmic reticulum and the nuclear pore complex. It hassimilarity to the yeast SEC13 protein, which is required forvesicle biogenesis from endoplasmic reticulum during the transportof proteins. Multiple alternatively spliced transcript variantshave been found.
Background Reference 1:
Nielsen, A.L. Biochem. Biophys. Res. Commun. 388(3):571-575(2009)
Background Reference 2:
Townley, A.K., et al. J. Cell. Sci. 121 (PT 18), 3025-3034 (2008) :
Background Reference 3:
Stagg, S.M., et al. Cell 134(3):474-484(2008)
Background Reference 4:
Hsia, K.C., et al. Cell 131(7):1313-1326(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-380

List Size:
400 ul

List Price:

$495.00

Datasheet
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