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Western blot analysis of SEC62 in rat brain tissue lysate with SEC62 antibody at (A) 0.5 and (B) 1 μg/mL.
Immunohistochemistry of SEC62 in mouse brain tissue with SEC62 antibody at 5 μg/mL.
Immunofluorescence of SEC62 in mouse brain tissue with SEC62 antibody at 20 μg/mL.
Western blot analysis of SEC62 in rat brain tissue lysate with SEC62 antibody at (A) 0.5 and (B) 1 μg/mL.
Trial Size

SEC62 Antibody

CATALOG NUMBER: 6471

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (100%)
Immunogen:
SEC62 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SEC62.
The immunogen is located within amino acids 270 - 320 of SEC62.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
SEC62 antibody can be used for detection of SEC62 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
SEC62 antibody is predicted to not cross-react with other SEC proteins. Multiple isoforms of SEC62 are known to exist.
Positive Control 1:
Cat. No. 1463 - Rat Brain Tissue Lysate
Purification:
SEC62 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SEC62 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SEC62 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SEC62
Additional Names:
SEC62 Antibody: HTP1, TP-1, Dtrp1, TLOC1, Translocation protein SEC62, Translocation protein 1
Protein Accession Number:
NP_003253
Protein Gi Number:
4507525
Ncbi Gene Id Number:
7095
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SEC62 Antibody: SEC62 is an integral membrane protein located in the rough endoplasmic reticulum (ER) and is part of the SEC61-SEC62-SEC63 complex that is the central component of the protein translocation apparatus of the ER membrane. It is speculated that SEC61-SEC62-SEC63 may perform post-translational protein translocation into the ER and might also perform the backward transport of ER proteins that are subject to the ubiquitin-proteasome-dependent degradation pathway. Silencing of this gene with RNAi dramatically reduced the migration and invasive potential of numerous tumor cell lines, suggesting that it may be an attractive target for therapy of various tumors.
Background Reference 1:
Deshaies RJ and Scheckman R. SEC62 encodes a putative membrane protein required for protein translocation in the yeast endoplasmic reticulum. J. Cell Biol. 1989; 109:2653-64.
Background Reference 2:
Meyer HA, Grau H, Kraft R, et al. Mammalian Sec61 is associated with Sec62 and Sec63. J. Biol. Chem. 2000; 275:14550-7
Background Reference 3:
Greiner M, Kreutzer B, Jung V, et al. Silencing of the SEC62 gene inhibits migratory and invasive potential of various tumor cells. Int. J. Cancer 2011; 128:2284-95.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6471

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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