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Western blot analysis of lysed extracts from HepG2 cells untreated or treated with EGF using SEK1/MKK4 (Phospho-Ser80).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SEK1/MKK4 (Phospho-Ser80).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using SEK1/MKK4 (Phospho-Ser80).
Western blot analysis of lysed extracts from HepG2 cells untreated or treated with EGF using SEK1/MKK4 (Phospho-Ser80).

SEK1 / MKK4 (phospho Ser80) Antibody

CATALOG NUMBER: 79-186

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
SEK1/MKK4 (Phospho-Ser80) antibody was raised against a peptide sequence around phosphorylation site of serine 80 (T-H-S (p) -I-E) derived from Human SEK1/MKK4.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of SEK1/MKK4 only when phosphorylated at serine 80.
Predicted Molecular Weight:
44 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
MAP2K4
Additional Names:
JNKK, MEK4, MKK4, SEK1, SKK1, JNKK1, SERK1, MAPKK4, PRKMK4, SAPKK1, SAPKK-1, SRK, JNKK, MAP2K4, MAPK/ERK kinase 4, MAPKK 4
Protein Accession Number:
NP_003001.1
Protein Gi Number:
4506889
Ncbi Gene Id Number:
6416
Background:
Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1).
Background Reference 1:
Park HS, et al. (2002) J Biol Chem; 277 (4): 2573-8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-186

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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