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Western blot analysis of SELK in A20 cell lysate with SELK antibody at (A) 1 and (B) 2 μg/mL.
Immunofluorescence of SELK in human heart tissue with SELK antibody at 20 μg/mL.
Western blot analysis of SELK in A20 cell lysate with SELK antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

SELK Antibody

CATALOG NUMBER: 6975

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Pig: (100%)
Immunogen:
SELK antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SELK.
The immunogen is located within the last 50 amino acids of SELK.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
SELK antibody can be used for detection of SELK by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1288 - A20 Cell Lysate
Positive Control 2:
Cat. No. 10-501 - Human Heart Tissue Slide
Predicted Molecular Weight:
10 kDa
Purification:
SELK Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SELK Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SELK antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SELK
Additional Names:
SELK Antibody: SelK, HSPC030, HSPC297, Selenoprotein K, SelK
Protein Accession Number:
NP_067060
Protein Gi Number:
25014099
Ncbi Gene Id Number:
58515
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SELK Antibody: SELK is a selenoprotein, containing a selenocysteine residue at its active site. SELK is localized to the endoplasmic reticulum and is highly expressed in the heart, where it may function as an antioxidant. SELK can bind to proteins such as the ER-associated degradation (ERAD) components p97 ATPase, Derlin-1 and -2, and SelS as well as proteins in the oligosaccharyltransferase complex, suggesting that SELK may be involved in the Derlin-dependent ERAD of glycosylated misfolded proteins. SELK is also thought to be important in Ca2+ flux in immune cells, and is cleaved by m-calpain in resting macrophages. When these macrophages are activated through different Toll-like receptors, this cleavage is inhibited
Background Reference 1:
Lu C, Qiu F, Zhou H, et al. Identification and characterization of selenoprotein K: An antioxidant in cardiomyocytes. FEBS Lett. 2006; 580:5189-97
Background Reference 2:
Shchedrina VA, Everly RA, Zhang Y, et al. Selenoprotein K binds multiprotein complexes and is involved in the regulation of endoplasmic homeostasis. J. Biol. Chem. 2011; 286:42937-48.
Background Reference 3:
Huang Z, Hoffmann FW, Norton RL, et al. Selenoprotein K is a novel target of m-calpain, and cleavage is regulated by Toll-like receptor-induced calpastatin in macrophages. J. Biol. Chem. 2011; 286:34830-8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6975

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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