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Western blot analysis in Jurkat cell line lysates (35ug/lane).
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in Jurkat cell line lysates (35ug/lane).

SELPLG Antibody

CATALOG NUMBER: 55-196

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This SELPLG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 379-405 amino acids from the C-terminal region of human SELPLG.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
43 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SELPLG
Additional Names:
P-selectin glycoprotein ligand 1, PSGL-1, Selectin P ligand, CD162, SELPLG
Protein Accession Number:
Q14242
Protein Gi Number:
2498904
Ncbi Gene Id Number:
6404
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SELPLG is the high affinity counter-receptor forP-selectin on myeloid cells and stimulated T lymphocytes. As such,it plays a critical role in the tethering of these cells toactivated platelets or endothelia expressing P-selectin. Theorganization of the SELPG gene closely resembles that of CD43 andthe human platelet glycoprotein GpIb-alpha both of which have anintron in the 5-prime-noncoding region, a long second exoncontaining the complete coding region, and TATA-less promoters.
Background Reference 1:
Elmas, E., et al. J. Cardiovasc. Electrophysiol. 21(11):1260-1265(2010)
Background Reference 2:
Luan, S.L., et al. J. Pathol. 222(2):166-179(2010)
Background Reference 3:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 4:
Russo, H.M., et al. Circ. Res. 107(3):388-397(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-196

List Size:
400 ul

List Price:

$495.00

Datasheet
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