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Western blot analysis in Hela cell line lysates (35ug/lane).
SELV Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of SELV Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis in Hela cell line lysates (35ug/lane).

SELV Antibody

CATALOG NUMBER: 55-794

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This SELV antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 296-324 amino acids from the C-terminal region of human SELV.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
37 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SELV
Additional Names:
Selenoprotein V, SelV, SELV
Protein Accession Number:
P59797
Protein Gi Number:
190358771
Ncbi Gene Id Number:
348303
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a selenoprotein, which contains aselenocysteine (Sec) residue at its active site. The selenocysteineis encoded by the UGA codon that normally signals translationtermination. The 3' UTR of selenoprotein genes have a commonstem-loop structure, the sec insertion sequence (SECIS), that isnecessary for the recognition of UGA as a Sec codon rather than asa stop signal.
Background Reference 1:
Kryukov, G.V., et al. Science 300(5624):1439-1443(2003)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-794

List Size:
400 ul

List Price:

$495.00

Datasheet
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