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Western blot analysis of SEMA3A Antibody in CEM cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with SEMA3A antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of SEMA3A Antibody in CEM cell line lysates (35ug/lane)

SEMA3A Antibody

CATALOG NUMBER: 63-186

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Chicken, Mouse, Rat
Immunogen:
This SEMA3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 535-566 amino acids from the Central region of human SEMA3A.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
89 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SEMA3A
Additional Names:
Semaphorin-3A, Semaphorin III, Sema III, SEMA3A, SEMAD
Protein Accession Number:
Q14563
Protein Gi Number:
8134674
Ncbi Gene Id Number:
10371
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SEMA3A is a member of the semaphorin family with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with the progression of Alzheimer's disease.
Background Reference 1:
Narazaki,M., Blood 111 (8), 4126-4136 (2008)
Background Reference 2:
Tannemaat,M.R., J. Neurosci. 27 (52), 14260-14264 (2007)
Background Reference 3:
Appleton,B.A., EMBO J. 26 (23), 4902-4912 (2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-186

List Size:
400 ul

List Price:

$495.00

Datasheet
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