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46-349 (4ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
46-349 (4ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Semaphorin 5A Antibody

CATALOG NUMBER: 46-349

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: DEATKKACYSKGKS
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave an approx 90kDa in Mouse Brain lysates and approx 25kDa band in Human Brain (Cerebellum), Colon, Heart and Kidney lysates after 0.03ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 121kDa according to NP_003957.1. The 90kDa and 25kDa band were successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:In paraffin embedded Human Kidney shows membanous and vesicular staining in PCT cells. Recommended concentration: 4-6ug/ml.
Specificity:
The immunizing peptide corresponds to a part of the extracellular domain.
Positive Control 1:
Cat. No. 1312 - Human Prostate Tissue Lysate
Positive Control 2:
Cat. No. 10-101 - Human Prostate Tissue Slide
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SEMA5A
Additional Names:
SEMA5A, sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A, HGNC:10736, SEMAF, semF, sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane
Protein Accession Number:
NP_003957.1
Protein Gi Number:
4506881
Ncbi Gene Id Number:
9037
Background Reference 1:
Maraganore DM, de Andrade M, Lesnick TG, Strain KJ, Farrer MJ, Rocca WA, Pant PV, Frazer KA, Cox DR, Ballinger DG. High-resolution whole-genome association study of Parkinson disease Am J Hum Genet. 2005 Nov;77(5):685-93. Epub 2005 Sep 9.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-349

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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