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Western blot analysis of SENP2 polyclonal antibody in HL-60 cell lysate
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot analysis of SENP2 polyclonal antibody in HL-60 cell lysate

SENP2 Antibody

CATALOG NUMBER: 56-359

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This SENP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 502-533 amino acids from the C-terminal region of human SENP2.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
68 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SENP2
Additional Names:
Sentrin-specific protease 2, Axam2, SMT3-specific isopeptidase 2, Smt3ip2, Sentrin/SUMO-specific protease SENP2, SENP2, KIAA1331
Protein Accession Number:
Q9HC62
Protein Gi Number:
143811458
Ncbi Gene Id Number:
59343
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SUMO is a small ubiquitin-like protein that can be covalently conjugated to other proteins. SENP2 is one of a group of enzymes that process newly synthesized SUMO1, SUMO2, and SUMO3 into the conjugatable mature forms and catalyze the deconjugation of these same SUMO proteins from their targeted substrates. SENP2 may also down-regulate CTNNB1 levels and thereby modulate the Wnt pathway.
Background Reference 1:
Zhang, H., et al., Mol. Cell. Biol. 22(18):6498-6508 (2002).
Background Reference 2:
Hang, J., et al., J. Biol. Chem. 277(22):19961-19966 (2002).
Background Reference 3:
Nishida, T., et al., J. Biol. Chem. 276(42):39060-39066 (2001).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-359

List Size:
400 ul

List Price:

$495.00

Datasheet
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