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Western blot analysis of SENP5 polyclonal antibody in Saos-2 cell lysate
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SENP5 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of SENP5 polyclonal antibody in Saos-2 cell lysate

SENP5 Antibody

CATALOG NUMBER: 56-385

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Monkey
Immunogen:
This SENP5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 524-555 amino acids from the C-terminal region of human SENP5.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
87 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SENP5
Additional Names:
Sentrin-specific protease 5, Sentrin/SUMO-specific protease SENP5, SENP5
Protein Accession Number:
Q96HI0
Protein Gi Number:
296452962
Ncbi Gene Id Number:
205564
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SENP5 is a protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO3 to its conjugatable mature form and deconjugation of SUMO2 and SUMO3 from targeted substrates. This protein has weak proteolytic activity against full-length SUMO1 or SUMO1 conjugates. SENP5 is required for cell division.
Background Reference 1:
Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-385

List Size:
400 ul

List Price:

$495.00

Datasheet
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