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SEPN1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in Hela,HepG2 cell line lysates (35ug/lane).
SEPN1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.

SEPN1 Antibody

CATALOG NUMBER: 56-434

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This SEPN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 417-445 amino acids from the C-terminal region of human SEPN1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For IHC-P starting dilution is: 1:10~50
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
66 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SEPN1
Additional Names:
Selenoprotein N, SelN, SEPN1, SELN
Protein Accession Number:
Q9NZV5
Protein Gi Number:
317373588
Ncbi Gene Id Number:
57190
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a selenoprotein, which contains aselenocysteine (Sec) residue at its active site. The selenocysteineis encoded by the UGA codon that normally signals translationtermination. The 3' UTR of selenoprotein genes have a commonstem-loop structure, the sec insertion sequence (SECIS), that isnecessary for the recognition of UGA as a Sec codon rather than asa stop signal. Mutations in this gene cause the classical phenotypeof multiminicore disease and congenital muscular dystrophy withspinal rigidity and restrictive respiratory syndrome. Twoalternatively spliced transcript variants encoding distinctisoforms have been found for this gene.
Background Reference 1:
Arbogast, S., et al. Ann. Neurol. 65(6):677-686(2009)
Background Reference 2:
Maiti, B., et al. Hum. Mutat. 30(3):411-416(2009)
Background Reference 3:
Jurynec, M.J., et al. Proc. Natl. Acad. Sci. U.S.A. 105(34):12485-12490(2008)
Background Reference 4:
Lin, L., et al. PLoS Genet.(10), E1000225 (2008) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-434

List Size:
400 ul

List Price:

$495.00

Datasheet
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