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Western blot analysis of anti-SEPSH2 Pabin HepG2 cell line lysate (35ug/lane).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot analysis of anti-SEPSH2 Pabin HepG2 cell line lysate (35ug/lane).

SEPSH2 Antibody

CATALOG NUMBER: 62-714

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This SEPSH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-201 amino acids from the Central region of human SEPSH2.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
47 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SEPHS2
Additional Names:
Selenide, water dikinase 2, Selenium donor protein 2, Selenophosphate synthase 2, SEPHS2, SPS2
Protein Accession Number:
Q99611
Protein Gi Number:
172044671
Ncbi Gene Id Number:
22928
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This protein encodes an enzyme that synthesizes selenophosphate from selenide and ATP. Selenophosphate is the selenium donor used to synthesize selenocysteine, which is co-translationally incorporated into selenoproteins at in-frame UGA codons. This protein itself contains a selenocysteine residue in its predicted active site. The 3' UTR of the gene has a stem-loop secondary structure called a selenocysteine insertion sequence (SECIS) element, which allows UGA to direct the incorporation of selenocysteine rather than signal a translational stop.
Background Reference 1:
Tamura, T., et al., Proc. Natl. Acad. Sci. U.S.A. 101(46):16162-16167 (2004).
Background Reference 2:
Lescure, A., et al., J. Biol. Chem. 274(53):38147-38154 (1999).
Background Reference 3:
Guimaraes, M.J., et al., Proc. Natl. Acad. Sci. U.S.A. 93(26):15086-15091 (1996).
Background Reference 4:
Guimaraes, M.J., et al., Development 121(10):3335-3346 (1995).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-714

List Size:
400 ul

List Price:

$495.00

Datasheet
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