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Western blot analysis of SEPT1 in Raji cell lysate with SEPT1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry of SEPT1 in Raji cells with SEPT1 antibody at 5 μg/mL.
Immunofluorescence of SEPT1 in Raji cells with SEPT1 antibody at 20 μg/mL.
Western blot analysis of SEPT1 in Raji cell lysate with SEPT1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Trial Size

SEPT1 Antibody

CATALOG NUMBER: 6903

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
SEPT1 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human SEPT1.
The immunogen is located within amino acids 300 - 350 of SEPT1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
SEPT1 antibody can be used for detection of SEPT1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least two isoforms of SEPT1 are known to exist. This antibody is predicted to not cross-react with other Septin protein family members.
Positive Control 1:
Cat. No. 1207 - Raji Cell Lysate
Positive Control 2:
Cat. No. 17-007 - Raji Cell Slide
Purification:
SEPT1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SEPT1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SEPT1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SEPT1
Additional Names:
SEPT1 Antibody: LARP, SEP1, DIFF6, PNUTL3, Septin-1, LARP
Protein Accession Number:
NP_443070
Protein Gi Number:
431822382
Ncbi Gene Id Number:
1731
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SEPT1 Antibody: SEPT1 (also known as Septin 1), is a member of the septin family of GTPases. Members of this family are required for cytokinesis and the maintenance of cellular morphology. SEPT1 was initially identified through yeast two-hybrid screening as an interaction partner for the serine/threonine kinase Aurora-B. During mitosis, SEPT1 localizes to the spindle pole, suggesting that it may play a role in cytokinesis and chromosome congression.
Background Reference 1:
Qi M, Yu W, Liu S, et al. Septin1, a new interaction partner for human serine/threonine kinase aurora-B. Biochem. Biophys. Res. Commun. 2005; 336:994-1000.
Background Reference 2:
Russell SE and Hall PA. Septin genomics: a road less travelled. Biol. Chem. 2011; 392:763-7.
Background Reference 3:
Zhu J, Qi ST, Wang YP, et al. Septin1 is required for spindle assembly and chromosome congression in mouse oocytes. Dev. Dyn. 2011; 240:2281-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6903

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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