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42-672 (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-672 (3.75ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-672 (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

SEPT4 Antibody

CATALOG NUMBER: 42-672

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-RNKLTRESGTD
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 50kDa band observed in Human and Mouse Heart lysates (calculated MW of 55.1kDa according to NP_004565.1 and 53.0kDa according to NP_536341). Recommended concentration: 1-3ug/ml.Immunohistochemistry:Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 3.75ug/ml.
Specificity:
This antibody is expected to recognize isoform 1 (NP_004565.1), 3 (NP_536341.1) and 4 (NP_001185642.1). Amino acid numbering in name refers to NP_004565.1 sequence.
Positive Control 1:
Cat. No. 1301 - Human Heart Tissue Lysate
Positive Control 2:
Cat. No. 1401 - Mouse Heart Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
41886
Additional Names:
SEPT4, septin 4, ARTS, BRADEION, CE5B3, H5, MART, PNUTL2, SEP4, hCDCREL-2, hucep-7, cell division control-related protein 2, cerebral protein 7, peanut-like 2, septin-M
Protein Accession Number:
NP_004565.1,NP_536341.1,NP_001185642.1
Protein Gi Number:
4758942
Ncbi Gene Id Number:
5414

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-672

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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