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Western blot analysis of extracts of various cell lines, using SEPT7 antibody (19-158).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat Intestine using SEPT7 antibody (19-158) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using SEPT7 antibody (19-158) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using SEPT7 antibody (19-158) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using SEPT7 antibody (19-158).
Western blot analysis of extracts of various cell lines, using SEPT7 antibody (19-158).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

SEPT7 Antibody

CATALOG NUMBER: 19-158

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 307-436 of human SEPT7 (NP_001011553.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:1000 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:100
Positive Control 1:
Jurkat
Positive Control 2:
HeLa
Positive Control 3:
A375
Positive Control 4:
HepG2
Positive Control 5:
Mouse testis
Positive Control 6:
Rat testis
Predicted Molecular Weight:
Observed: 51kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SEPT7.
Additional Names:
SEPT7, septin 7, CDC10, CDC3, Nbla02942, SEPT7A, CDC10 (cell division cycle 10, S. cerevisiae, homolog), CDC10 cell division cycle 10 homolog, cell division cycle 10
Ncbi Gene Id Number:
989
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-158

List Size:
100 uL

List Price:

$436.00

Datasheet
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