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43-428 (0.1ug/ml) staining of Human (A), Mouse (B) and Rat (C) Heart lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-428 (5ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-428 (0.1ug/ml) staining of Human (A), Mouse (B) and Rat (C) Heart lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.

SERCA2 / ATP2A2 Antibody

CATALOG NUMBER: 43-428

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig
Immunogen:
The immunogen for this antibody is: DELNPSAQRDACLN
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 110kDa band observed in Human, Mouse and Rat Heart lysates (calculated MW of 110kDa according to NP_001672.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Heart. Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_001672.1; NP_733765.1).
Predicted Molecular Weight:
Approx 110kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SERCA2 / ATP2A2
Additional Names:
ATP2A2, ATPase, Ca++ transporting, cardiac muscle, slow twitch 2, ATP2B, DAR, DD, DKFZp686P0211, FLJ20293, FLJ38063, MGC45367, SERCA2, ATPase, Ca++ dependent, slow-twitch, cardiac muscle-2, SR Ca(2+)-ATPase 2, calcium pump 2, calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform, cardiac Ca2+ ATPase, endoplasmic reticulum class 1/2 Ca(2+) ATPase, sarcoplasmic/endoplasmic reticulum calcium ATPase 2
Protein Accession Number:
NP_001672.1,NP_733765.1
Protein Gi Number:
4502285
Ncbi Gene Id Number:
488
Background Reference 1:
Satoh K, Matsu-Ura T, Enomoto M, Nakamura H, Michikawa T, Mikoshiba K.Highly cooperative dependence of sarco/endoplasmic reticulum calcium ATPase SERCA2a pump activity on cytosolic calcium in living cells.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-428

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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