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46-357 (0.3ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-357 (3.75ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-357 (0.3ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Serotonin receptor 3A Antibody

CATALOG NUMBER: 46-357

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Rat
Immunogen:
The immunogen for this antibody is: C-GPQDFEKSPRDR
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 55kDa band observed in Human Colon lysates (calculated MW of 55.3kDa according to NP_000860.2. Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 200kDa. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:Paraffin embedded Human Small Intestine. Recommended concentration: 3.75ug/ml. ICC/ IF:This product has been successfully used in IF/ ICC on Rat: Lu CL et al, Gastroenterology. 2009 Sep;137(3):1040-50. PMID: 19344720.
Specificity:
This antibody is expected to recognise all three reported isoforms (NP_998786.2; NP_000860.2; NP_001155244.1).
Positive Control 1:
Cat. No. 1320 - Human Colon Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
HTR3A
Additional Names:
HTR3A, serotonin receptor, 5-hydroxytryptamine (serotonin) receptor 3A, 5-HT-3, 5-HT3A, 5-HT3R, 5HT3R, HTR3, 5-hydroxytryptamine (serotonin) receptor-3, 5HT3 serotonin receptor, Serotonin-gated ion channel receptor, truncated receptor, containing only 3 transmembrane domains
Protein Accession Number:
NP_998786.2,NP_000860.2,NP_001155244.1
Protein Gi Number:
47519841
Ncbi Gene Id Number:
3359
Background Reference 1:
Dremencov E, Weizmann Y, Kinor N, Gispan-Herman I, Yadid G. Modulation of dopamine transmission by 5HT2C and 5HT3 receptors: a role in the antidepressant response. Curr Drug Targets. 2006 Feb;7(2):165-75. Review.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-357

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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