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Western blot analysis of extracts of various cell lines, using SERPINA1 antibody (14-451) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human liver cancer using SERPINA1 antibody (14-451) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using SERPINA1 antibody (14-451) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of PC-12 cells using SERPINA1 antibody (14-451) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using SERPINA1 antibody (14-451) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

SERPINA1 Antibody

CATALOG NUMBER: 14-451

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 25-315 of human SERPINA1 (NP_000286.3).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
SW480
Positive Control 2:
HT-29
Positive Control 3:
Mouse lung
Predicted Molecular Weight:
Observed: 45kDa,50kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SERPINA1
Additional Names:
SERPINA1, PI, A1A, AAT, PI1, A1AT, PRO2275, alpha1AT
Ncbi Gene Id Number:
5265
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-451

List Size:
100 uL

List Price:

$436.00

Datasheet
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