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Western blot analysis of extracts of HepG2 cells, using SERPINA10 antibody (19-451) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 120s.
Immunofluorescence analysis of A549 cells using SERPINA10 antibody (19-451). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using SERPINA10 antibody (19-451) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of HepG2 cells, using SERPINA10 antibody (19-451) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 120s.

SERPINA10 Antibody

CATALOG NUMBER: 19-451

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 165-444 of human SERPINA10 (NP_001094077.1).
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
WB: 1:500 - 1:2000
IF: 1:20 - 1:100
Positive Control 1:
HepG2
Predicted Molecular Weight:
Observed: 51kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SERPINA10
Additional Names:
SERPINA10, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 10, PZI, ZPI, protein Z-dependent protease inhibitor, protein Z-dependent protease inhibitorprecursor, serine (or cysteine) proteinase inhibitor, clade A (alph, UNQ707/PRO1358
Ncbi Gene Id Number:
51156
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene belongs to the serpin family. It is predominantly expressed in the liver and secreted in plasma. It inhibits the activity of coagulation factors Xa and XIa in the presence of protein Z, calcium and phospholipid. Mutations in this gene are associated with venous thrombosis. Alternatively spliced transcript variants have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-451

List Size:
100 uL

List Price:

$436.00

Datasheet
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