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Western blot analysis of extracts of various cell lines, using SERPINA3 antibody (18-819) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat liver using SERPINA3 antibody (18-819) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using SERPINA3 antibody (18-819) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using SERPINA3 antibody (18-819) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using SERPINA3 antibody (18-819) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.

SERPINA3 Antibody

CATALOG NUMBER: 18-819

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 24-423 of human SERPINA3 (NP_001076.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:200 - 1:1000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
HepG2
Positive Control 2:
Mouse liver
Positive Control 3:
Rat liver
Predicted Molecular Weight:
Observed: 48kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SERPINA3
Additional Names:
Alpha-1-antichymotrypsin, ACT, Cell growth-inhibiting gene 24/25 protein, Serpin A3, Alpha-1-antichymotrypsin His-Pro-less, SERPINA3, AACT
Ncbi Gene Id Number:
12
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a plasma protease inhibitor and member of the serine protease inhibitor class. Polymorphisms in this protein appear to be tissue specific and influence protease targeting. Variations in this protein's sequence have been implicated in Alzheimer's disease, and deficiency of this protein has been associated with liver disease. Mutations have been identified in patients with Parkinson disease and chronic obstructive pulmonary disease.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-819

List Size:
100 uL

List Price:

$436.00

Datasheet
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