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IHC testing of FFPE human pancreas with Alpha-1-Antichymotrypsin antibody (clone AACT/1452). Required HIER: steam sections in pH6, 10mM citrate buffer for 10-20 min.
SDS-PAGE Analysis of Purified, BSA-Free Alpha-1-Antichymotrypsin Antibody (clone AACT/1452). Confirmation of Integrity and Purity of the Antibody.
IHC testing of FFPE human pancreas with Alpha-1-Antichymotrypsin antibody (clone AACT/1452). Required HIER: steam sections in pH6, 10mM citrate buffer for 10-20 min.

SERPINA3 Antibody [AACT/1452]

CATALOG NUMBER: 33-604

Clonality:
Monoclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
A human partial recombinant protein was used as the immunogen for this Alpha-1-Antichymotrypsin antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
Western blot: 0.5-1 ug/ml
IHC (Paraffin): 0.5-1 ug/ml
IF: 0.5-1 ug/ml
Flow Cytometry: 0.5-1ug/10e6 cells in 0.1ml
The stated application concentrations are suggested starting amounts. Titration of the Alpha-1-Antichymotrypsin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Purification:
Protein G affinity
Clonality:
Monoclonal
Isotype:
IgG1
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
SERPINA3
Additional Names:
Alpha-1-antichymotrypsin, ACT, Cell growth-inhibiting gene 24/25 protein, Serpin A3, Alpha-1-antichymotrypsin His-Pro-less, SERPINA3, AACT
Ncbi Gene Id Number:
12
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Alpha 1-Antichymotrypsin, also called AACT and SERPINA3, inhibits the activity of certain proteases, such as cathepsin G that is found in neutrophils, and chymases found in mast cells, by cleaving them into a different shape or conformation. This activity protects some tissues, such as the lower respiratory tract, from damage caused by proteolytic enzymes. This protein is produced in the liver, and is an acute phase protein that is induced during inflammation. [Wiki]

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-604

List Size:
100 ug

List Price:

$626.00

Datasheet
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