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43-512 (1ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-512 (5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-512 (1ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

SERPINB1 Antibody

CATALOG NUMBER: 43-512

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-EVHSRFQSLNADINKR
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 43kDa band observed in lysates of cell line A549 (calculated MW of 42.7kDa according to NP_109591.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Spleen (neutrophils). Recommended concentration: 5ug/ml.
Predicted Molecular Weight:
Approx 43kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SERPINB1
Additional Names:
SERPINB1, serpin peptidase inhibitor, clade B (ovalbumin), member 1, EI, ELANH2, LEI, M/NEI, MNEI, PI-2, PI2, leukocyte elastase inhibitor, peptidase inhibitor 2, protease inhibitor 2 (anti-elastase), monocyte/neutrophil derived, serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 1
Protein Accession Number:
NP_109591.1
Protein Gi Number:
13489087
Ncbi Gene Id Number:
1992
Background Reference 1:
Cooley J, Sontag MK, Accurso FJ, Remold-O'Donnell E.SerpinB1 in cystic fibrosis airway fluids: quantity, molecular form and mechanism of elastase inhibition.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-512

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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