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Western blot analysis of SERPINE1 using rabbit polyclonal SERPINE1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SERPINE1 gene.
Flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of SERPINE1 using rabbit polyclonal SERPINE1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SERPINE1 gene.

SERPINE1 Antibody

CATALOG NUMBER: 56-733

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This SERPINE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188-216 amino acids from the Central region of human SERPINE1.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
45 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SERPINE1
Additional Names:
Plasminogen activator inhibitor 1, PAI, PAI-1, Endothelial plasminogen activator inhibitor, Serpin E1, SERPINE1, PAI1, PLANH1
Protein Accession Number:
P05121
Protein Gi Number:
129576
Ncbi Gene Id Number:
5054
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the serine proteinaseinhibitor (serpin) superfamily. This member is the principalinhibitor of tissue plasminogen activator (tPA) and urokinase(uPA), and hence is an inhibitor of fibrinolysis. Defects in thisgene are the cause of plasminogen activator inhibitor-1 deficiency(PAI-1 deficiency), and high concentrations of the gene product areassociated with thrombophilia. Alternatively spliced transcriptvariants encoding different isoforms have been found for this gene.
Background Reference 1:
Ma, Z., et al. Biochem. Biophys. Res. Commun. 400(4):569-574(2010)
Background Reference 2:
Ince, D.A., et al. Genet Test Mol Biomarkers 14(5):643-647(2010)
Background Reference 3:
Bern, M.M., et al. Clin. Appl. Thromb. Hemost. 16(5):574-578(2010)
Background Reference 4:
Markl, B., et al.Surg Oncol 102(3):235-241(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-733

List Size:
400 ul

List Price:

$495.00

Datasheet
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