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Western blot analysis of SERPINH1 antibody in Hela cell line lysates (35ug/lane)
SERPINH1 Antibody (RB18953) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of SERPINH1 antibody in Hela cell line lysates (35ug/lane)

SERPINH1 Antibody

CATALOG NUMBER: 62-857

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Mouse, Rat
Immunogen:
This SERPINH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 390-418 amino acids from the C-terminal region of human SERPINH1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
46 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SERPINH1
Additional Names:
Serpin H1, 47 kDa heat shock protein, Arsenic-transactivated protein 3, AsTP3, Cell proliferation-inducing gene 14 protein, Collagen-binding protein, Colligin, Rheumatoid arthritis-related antigen RA-A47, SERPINH1, CBP1, CBP2, HSP47, SERPINH2
Protein Accession Number:
P50454
Protein Gi Number:
20141241
Ncbi Gene Id Number:
871
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SERPINH1 is a member of the serpin superfamily of serine proteinase inhibitors. Its expression is induced by heat shock. The protein localizes to the endoplasmic reticulum lumen and binds collagen; thus it is thought to be a molecular chaperone involved in the maturation of collagen molecules. Autoantibodies to this protein have been found in patients with rheumatoid arthritis.
Background Reference 1:
Araki,K., Br. J. Cancer 101 (3), 492-497 (2009)
Background Reference 2:
Katagata,Y., J. Dermatol. Sci. 49 (1), 33-38 (2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-857

List Size:
400 ul

List Price:

$495.00

Datasheet
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