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Recombinant Serratia marcescens Nuclease was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
One unit (U) is defined as the amount of enzyme required to change the absorption value of △A260 by 1.0 (equivalent to complete digestion of 37 μg of salmon essence DNA into oligonucleotides) in 30 min at 37°C, pH 8.0 reaction conditions. The specific activity of Serratia marcescens nuclease is > 1000 U/μL.
Recombinant Serratia marcescens Nuclease (0.1U) can effectively degrade 5 μg plasmid DNA at 37°C for 30 min. The reaction buffer is: 10 mM MgCl2, 0.1 mg/mL BSA, 50 mM Tris-HCl, pH8.5.
Recombinant Serratia marcescens Nuclease was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Serratia Marcescens Nuclease (86-076)

CATALOG NUMBER: 86-076

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
Pichia
Species:
Serratia marcescens
source-species:
Pichia
Recombinant Protein Sequence:
Asp22-Asn266
Fusion Tag:
No tag
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
26.71 kDa
Biological Activity:
1.One unit (U) is defined as the amount of enzyme required to change the absorption value of △A260 by 1.0 (equivalent to complete digestion of 37 μg of salmon essence DNA into oligonucleotides) in 30 min at 37°C, pH 8.0 reaction conditions. The specific activity of Serratia marcescens nuclease is > 1000 U/μL.
2.Recombinant Serratia marcescens Nuclease (0.1U) can effectively degrade 5 μg plasmid DNA at 37°C for 30 min. The reaction buffer is: 10 mM MgCl2, 0.1 mg/mL BSA, 50 mM Tris-HCl, pH8.5.
Purity:
≥ 95 % as determined by SDS-PAGE.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
physical-state:
Lyophilized
Buffer:
Solution in 50% glycerol containing 20 mM Tris HCl, pH 8.0, 2 mM MgCl2, and 20 mM NaCl.
Storage Conditions:
Store at -70°C.This product is stable at ≤ -20°C for up to 1 year from the date of receipt.
For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.
Additional Names:
Endonuclease; Nuclease; nucA; nuc
Protein Accession Number:
WP_015377376.1
Ncbi Gene Id Number:
87005285
Background:
Catalyzes the hydrolysis of both DNA and RNA, double- or single-stranded, at the 3'position of the phosphodiester bond to produce 5'-phosphorylated mono-, di-, tri- and tetranucleotides. DNA is a slightly better substrate than RNA.

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

86-076

List Size:
50KU, 100KU

List Price:

Price range: $238.00 through $378.00

Datasheet
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