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Western blot analysis of SESTD1 in rat brain tissue lysate with SESTD1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of SESTD1 in human brain tissue with SESTD1 antibody at 5 μg/mL.
Immunofluorescence of SESTD1 in Human Brain cells with SESTD1 antibody at 20 μg/mL.
Western blot analysis of SESTD1 in rat brain tissue lysate with SESTD1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

SESTD1 Antibody

CATALOG NUMBER: 5877

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
SESTD1 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human SESTD1.
The immunogen is located within the last 50 amino acids of SESTD1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
SESTD1 antibody can be used for detection of SESTD1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1463 - Rat Brain Tissue Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
SESTD1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SESTD1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SESTD1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SESTD1
Additional Names:
SESTD1 Antibody: SOLO, SOLO, SEC14 domain and spectrin repeat-containing protein 1, Huntingtin-interacting protein-like protein
Protein Accession Number:
NP_835224
Protein Gi Number:
188528641
Ncbi Gene Id Number:
91404
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SESTD1 Antibody: SESTD1 was initially identified in mutant zebrafish with defects in the spontaneous contraction and touch response as a novel gene, solo, encoding a protein containing SEC14 and spectrin repeat domains. Other experiments indicated that SESTD1 interacts with the TRPC4 and TRPC5, members of the transient receptor potential channel family, via the TRPC calmodulin- and inositol 1, 4, 5-triphosphate receptor-binding domain and is essential for efficient receptor-mediated activation of TRPC5, suggesting that SESTD1 is a novel regulator of these TRPC proteins.
Background Reference 1:
Sato T and Mishina M. Representational difference analysis, high-resolution physical mapping, and transcript identification of the zebrafish genomic region for a motor behavior. Genomics2003; 82:218-29.
Background Reference 2:
Miehe S, Bieberstein A, Arnold I, et al. The phospholipid-binding protein SESTD1 is a novel regulator of the transient receptor potential channels TRPC4 and TRPC5. J. Biol. Chem.2010; 285:12426-34.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5877

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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