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Western blot analysis of extracts of various cell lines, using SET antibody (14-466) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat liver using SET antibody (14-466) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using SET antibody (14-466) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using SET antibody (14-466) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using SET antibody (14-466) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using SET antibody (14-466) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using SET antibody (14-466) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using SET antibody (14-466) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using SET antibody (14-466) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug BAX antibody (14-223). Western blot was performed from the immunoprecipitate using BAX antibody (14-223) at a dilition of 1:1000.
Immunohistochemistry of paraffin-embedded mouse kidney using SET antibody (14-466) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using SET antibody (14-466) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using SET antibody (14-466) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

SET Antibody

CATALOG NUMBER: 14-466

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 146-277 of human SET (NP_003002.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
HeLa
Positive Control 2:
293T
Positive Control 3:
BT-474
Positive Control 4:
HL-60
Positive Control 5:
NIH/3T3
Positive Control 6:
Mouse spleen
Predicted Molecular Weight:
Observed: 34kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SET
Additional Names:
SET, SET translocation (myeloid leukemia-associated), 2PP2A, I2PP2A, PHAPII, TAF-IBETA, SET, translocation, IGAAD, inhibitor-2 of protein phosphatase-2A, Inhibitor of GZMA-activated DNAse, TAF-I, IPP2A2
Ncbi Gene Id Number:
6418
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene inhibits acetylation of nucleosomes, especially histone H4, by histone acetylases (HAT). This inhibition is most likely accomplished by masking histone lysines from being acetylated, and the consequence is to silence HAT-dependent transcription. The encoded protein is part of a complex localized to the endoplasmic reticulum but is found in the nucleus and inhibits apoptosis following attack by cytotoxic T lymphocytes. This protein can also enhance DNA replication of the adenovirus genome. Several transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-466

List Size:
100 uL

List Price:

$419.00

Out of stock

Datasheet
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