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46-361 (1.5ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-361 (1.5ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

SETDB2 Antibody

CATALOG NUMBER: 46-361

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: GEKNGDAKTFWME-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 80kDa band observed in Human Heart lysates (calculated MW of 81.9kDa according to NP_114121.1). Recommended concentration: 1.5-4.5ug/ml.This product is not suitable in WB on Mouse Heart and Rat Heart lysates.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_114121.2; NP_001153780.1).
Positive Control 1:
Cat. No. 1301 - Human Heart Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SETDB2
Additional Names:
SETDB2, CLLD8, SET domain, bifurcated 2, CLLL8 protein, C13orf4, CLLL8, DKFZp586I0123, DKFZp761J1217, KMT1F, chronic lymphocytic leukemia deletion region 8, lysine N-methyltransferase 1F, histone-lysine N-methyltransferase SETDB2
Protein Accession Number:
NP_114121.2,NP_001153780.1
Protein Gi Number:
13994282
Ncbi Gene Id Number:
83852
Background Reference 1:
Mabuchi H, Fujii H, Calin G, Alder H, Negrini M, Rassenti L, Kipps TJ, Bullrich F, Croce CM. Cloning and characterization of CLLD6, CLLD7, and CLLD8, novel candidate genes for leukemogenesis at chromosome 13q14, a region commonly deleted in B-cell chronic lymphocytic leukemia. Cancer Res. 2001 Apr 1;61(7):2870-7.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-361

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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