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Western blot analysis of extracts from HUVEC cells treated with anisomycin using SF1 (Phospho-Ser82) Antibody. The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using SF1 (Phospho-Ser82) antibody (left) or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from HUVEC cells treated with anisomycin using SF1 (Phospho-Ser82) Antibody. The lane on the right is treated with the antigen-specific peptide.

SF1 (phospho Ser82) Antibody

CATALOG NUMBER: 79-794

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
SF1 (phospho Ser82) antibody was raised against a peptide sequence around phosphorylation site of Serine 82 (S-P-S (p)-P-E) derived from Human SF1.
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Specificity:
The antibody detects endogenous levels of SF1 only when phosphorylated at serine 82.
Predicted Molecular Weight:
68 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
SF1
Additional Names:
BBP, CW17, SF01, ZFM1, ZNF162
Protein Accession Number:
NP_004621.2
Protein Gi Number:
42544130
Ncbi Gene Id Number:
7536
Background:
Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor.
Background Reference 1:
Susan Y. Park, Development, May 2005; 132: 2415 - 2423.
Background Reference 2:
Dujin Zhou, Mol. Endocrinol., Jul 2000; 14: 986.
Background Reference 3:
Dagmar Wilhelm, Genes & Dev., Jul 2002; 16: 1839.
Background Reference 4:
Philipp Gut,Development, Oct 2005; 132: 4611 - 4619.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-794

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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