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Antibody used in WB on Human Placenta at 0.2-1 ug/ml.
Antibody used in WB on Human Placenta at 0.2-1 ug/ml.

SF3B14 Antibody

CATALOG NUMBER: 29-428

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SF3B14.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
SF3B14 antibody can be used for detection of SF3B14 by ELISA at 1:62500. SF3B14 antibody can be used for detection of SF3B14 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1309 - Human Placenta Lysate
Predicted Molecular Weight:
14 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store SF3B14 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
SF3B14
Additional Names:
SF3B14, CGI-110, HSPC175, Ht006, P14, SAP14, SF3B14a, SAP14a, SF3B14
Protein Accession Number:
NP_057131
Protein Gi Number:
7706326
Ncbi Gene Id Number:
51639
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SF3B14 is a 14 kDa protein subunit of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. This 14 kDa protein interacts directly with subunit 1 of the splicing factor 3b complex. SF3B14 also interacts directly with the adenosine that carries out the first transesterification step of splicing at the pre-mRNA branch site.This gene encodes a 14 kDa protein subunit of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. This 14 kDa protein interacts directly with subunit 1 of the splicing factor 3b complex. This 14 kDa protein also interacts directly with the adenosine that carries out the first transesterification step of splicing at the pre-mRNA branch site.
Background Reference 1:
Ewing, R.M., Mol. Syst. Biol. 3, 89 (2007).

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

29-428

List Size:
100 ul

List Price:

$519.00

Datasheet
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