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Western Blot at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: A549 whole cell lysate Lane 4: Hela whole cell lysate Lane 5: mouse spleen lysate Lane 6: mouse testis lysate Lane 7: rat lung lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: A549 whole cell lysate Lane 4: Hela whole cell lysate Lane 5: mouse spleen lysate Lane 6: mouse testis lysate Lane 7: rat lung lysate Lysates/proteins at 20 ug per lane.

SF3B2 Antibody

CATALOG NUMBER: 58-322

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
This SF3B2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 539-568 amino acids from the Central region of human SF3B2.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:2000
Predicted Molecular Weight:
100 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SF3B2
Additional Names:
Splicing factor 3B subunit 2, Pre-mRNA-splicing factor SF3b 145 kDa subunit, SF3b145, SF3b150, Spliceosome-associated protein 145, SAP 145, SF3B2, SAP145
Protein Accession Number:
Q13435
Protein Gi Number:
296452908
Ncbi Gene Id Number:
10992
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes subunit 2 of the splicing factor 3bprotein complex. Splicing factor 3b, together with splicing factor3a and a 12S RNA unit, forms the U2 small nuclearribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3acomplex binds pre-mRNA upstream of the intron's branch site in asequence-independent manner and may anchor the U2 snRNP to thepre-mRNA. Splicing factor 3b is also a component of the minorU12-type spliceosome. Subunit 2 associates with pre-mRNA upstreamof the branch site at the anchoring site. Subunit 2 also interactsdirectly with subunit 4 of the splicing factor 3b complex. Subunit2 is a highly hydrophilic protein with a proline-rich N-terminusand a glutamate-rich stretch in the C-terminus. [provided byRefSeq].
Background Reference 1:
Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
Background Reference 2:
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Background Reference 3:
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Background Reference 4:
Olsen, J.V., et al. Cell 127(3):635-648(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-322

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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