Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in MDA-MB453,Jurkat cell line lysates (35ug/lane).
Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.

SF3B3 Antibody

CATALOG NUMBER: 56-592

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse
Immunogen:
This SF3B3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1046-1074 amino acids from the C-terminal region of human SF3B3.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
136 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SF3B3
Additional Names:
Splicing factor 3B subunit 3, Pre-mRNA-splicing factor SF3b 130 kDa subunit, SF3b130, STAF130, Spliceosome-associated protein 130, SAP 130, SF3B3, KIAA0017, SAP130
Protein Accession Number:
Q15393
Protein Gi Number:
116242787
Ncbi Gene Id Number:
23450
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes subunit 3 of the splicing factor 3bprotein complex. Splicing factor 3b, together with splicing factor3a and a 12S RNA unit, forms the U2 small nuclearribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3acomplex binds pre-mRNA upstream of the intron's branch site in asequence independent manner and may anchor the U2 snRNP to thepre-mRNA. Splicing factor 3b is also a component of the minorU12-type spliceosome. Subunit 3 has also been identified as acomponent of the STAGA (SPT3-TAF(II)31-GCN5L acetylase)transcription coactivator-HAT (histone acetyltransferase) complex,and the TFTC (TATA-binding-protein-free TAF(II)-containingcomplex). These complexes may function in chromatin modification,transcription, splicing, and DNA repair.
Background Reference 1:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 2:
Will, C.L., et al. RNA 10(6):929-941(2004)
Background Reference 3:
Cavusoglu, N., et al. Proteomics 3(2):217-223(2003)
Background Reference 4:
Will, C.L., et al. EMBO J. 21(18):4978-4988(2002)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-592

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369