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Western blot analysis in Hela cell line lysates (35ug/lane).
Western blot analysis in Hela cell line lysates (35ug/lane).

SF3B4 Antibody

CATALOG NUMBER: 60-518

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This SF3B4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 158-187 amino acids from the Central region of human SF3B4.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
44 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SF3B4
Additional Names:
Splicing factor 3B subunit 4, Pre-mRNA-splicing factor SF3b 49 kDa subunit, SF3b50, Spliceosome-associated protein 49, SAP 49, SF3B4, SAP49
Protein Accession Number:
Q15427
Protein Gi Number:
2500587
Ncbi Gene Id Number:
10262
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes one of four subunits of the splicingfactor 3B. The protein encoded by this gene cross-links to a regionin the pre-mRNA immediately upstream of the branchpoint sequence inpre-mRNA in the prespliceosomal complex A. It also may be involvedin the assembly of the B, C and E spliceosomal complexes. Inaddition to RNA-binding activity, this protein interacts directlyand highly specifically with subunit 2 of the splicing factor 3B.This protein contains two N-terminal RNA-recognition motifs (RRMs),consistent with the observation that it binds directly to pre-mRNA.
Background Reference 1:
Gudbjartsson, D.F., et al. Nat. Genet. 40(5):609-615(2008)
Background Reference 2:
Rikova, K., et al. Cell 131(6):1190-1203(2007)
Background Reference 3:
Watanabe, H., et al. J. Biol. Chem. 282(28):20728-20738(2007)
Background Reference 4:
Wu, C., et al. Proteomics 7(11):1775-1785(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-518

List Size:
400 ul

List Price:

$495.00

Datasheet
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