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Antibody used in IHC on Human Muscle at 4.0-8.0 ug/ml.
Antibody used in WB on Human Jurkat cells at 5.0 ug/ml.
Antibody used in IHC on Human Muscle at 4.0-8.0 ug/ml.

SFPQ Antibody

CATALOG NUMBER: 29-345

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SFPQ.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
SFPQ antibody can be used for detection of SFPQ by ELISA at 1:1562500. SFPQ antibody can be used for detection of SFPQ by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
78 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store SFPQ antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
SFPQ
Additional Names:
SFPQ, POMP100, PSF
Protein Accession Number:
NP_005057
Protein Gi Number:
4826998
Ncbi Gene Id Number:
6421
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SFPQ is DNA- and RNA binding protein. It is essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as an heteromer with NONO. It binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. It interacts with U5 snRNA. It may be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. SFPQ may be involved in homologous DNA pairing. SFPQ is involved in transcriptional regulation. It binds the DNA sequence 5'-CTGAGTC-3' in the insulin-like growth factor response element (IGFRE) and inhibits IGF-I-stimulated transcriptional activity.
Background Reference 1:
Rosonina, E., (2005) Mol. Cell. Biol. 25 (15), 6734-6746.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

29-345

List Size:
100 ul

List Price:

$473.00

Datasheet
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