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Western blot analysis in 293 cell line lysates (35ug/lane).
SFRP2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in 293 cell line lysates (35ug/lane).

SFRP2 Antibody

CATALOG NUMBER: 56-366

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This SFRP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 263-292 amino acids from the C-terminal region of human SFRP2.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
33 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SFRP2
Additional Names:
Secreted frizzled-related protein 2, FRP-2, sFRP-2, Secreted apoptosis-related protein 1, SARP-1, SFRP2, FRP2, SARP1
Protein Accession Number:
Q96HF1
Protein Gi Number:
61216828
Ncbi Gene Id Number:
6423
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the SFRP family thatcontains a cysteine-rich domain homologous to the putativeWnt-binding site of Frizzled proteins. SFRPs act as solublemodulators of Wnt signaling. Methylation of this gene is apotential marker for the presence of colorectal cancer. [providedby RefSeq].
Background Reference 1:
von Marschall, Z., et al. Biochem. Biophys. Res. Commun. 400(3):299-304(2010)
Background Reference 2:
Pehlivan, S., et al. Cancer Genet. Cytogenet. 201(2):128-132(2010)
Background Reference 3:
Kohno, H., et al. Oncol. Rep. 24(2):423-431(2010)
Background Reference 4:
Yamamura, S., et al. Mol. Cancer Ther. 9(6):1680-1687(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-366

List Size:
400 ul

List Price:

$495.00

Datasheet
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