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42-066 (0.5ug/ml) staining of Human Uterus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-066 (2ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
42-066 (0.5ug/ml) staining of Human Uterus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

SFRP4 Antibody

CATALOG NUMBER: 42-066

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Cow, Pig
Immunogen:
The immunogen for this antibody is: TNSSCQCPHILPHQD
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 40kDa band observed in Human Uterus lysates (calculated MW of 39.8kDa according to NP_003005.2). Recommended concentration: 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Uterus shows granular staining in the apical cytoplasm of the endometrial gland. Recommended concentration, 2-4ug/ml.
Positive Control 1:
Cat. No. 1317 - Human Uterus Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SFRP4
Additional Names:
SFRP4, secreted frizzled-related protein 4, FRP-4, FRPHE, MGC26498, secreted frizzled-related protein 4
Protein Accession Number:
NP_003005.2
Protein Gi Number:
170784838
Ncbi Gene Id Number:
6424
Background Reference 1:
Linhart HG, Lin H, Yamada Y, Moran E, Steine EJ, Gokhale S, Lo G, Cantu E, Ehrich M, He T, Meissner A, Jaenisch R. Dnmt3b promotes tumorigenesis in vivo by gene-specific de novo methylation and transcriptional silencing. Genes Dev. 2007 Dec 1;21(23):3110-22.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-066

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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