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Western blot analysis in K562 cell line lysates (35ug/lane).
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in K562 cell line lysates (35ug/lane).

SFRP4 Antibody

CATALOG NUMBER: 55-922

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This SFRP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 318-346 amino acids from the C-terminal region of human SFRP4.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
40 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SFRP4
Additional Names:
Secreted frizzled-related protein 4, sFRP-4, Frizzled protein, human endometrium, FrpHE, SFRP4, FRPHE
Protein Accession Number:
Q6FHJ7
Protein Gi Number:
61216780
Ncbi Gene Id Number:
6424
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Secreted frizzled-related protein 4 (SFRP4) is a member ofthe SFRP family that contains a cysteine-rich domain homologous tothe putative Wnt-binding site of Frizzled proteins. SFRPs act assoluble modulators of Wnt signaling. The expression of SFRP4 inventricular myocardium correlates with apoptosis related geneexpression.
Background Reference 1:
Lee, D.Y., et al. Menopause 17(5):1064-1070(2010)
Background Reference 2:
Kohno, H., et al. Oncol. Rep. 24(2):423-431(2010)
Background Reference 3:
Frank, B., et al. Carcinogenesis 31(8):1381-1386(2010)
Background Reference 4:
Huang, D., et al. J. Cancer Res. Clin. Oncol. 136(3):395-401(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-922

List Size:
400 ul

List Price:

$495.00

Datasheet
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