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Western blot analysis of SFRS1 Antibody in HL-60 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human colon carcinoma reacted with SFRS1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of SFRS1 using rabbit polyclonal SFRS1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SFRS1 gene.
Flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of SFRS1 Antibody in HL-60 cell line lysates (35ug/lane)

SFRS1 Antibody

CATALOG NUMBER: 62-506

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Zebrafish, Bovine, Chicken, Mouse, Pig
Immunogen:
This SFRS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 158-186 amino acids from the C-terminal region of human SFRS1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
28 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SRSF1
Additional Names:
Serine/arginine-rich splicing factor 1, Alternative-splicing factor 1, ASF-1, Splicing factor, arginine/serine-rich 1, pre-mRNA-splicing factor SF2, P33 subunit, SRSF1, ASF, SF2, SF2P33, SFRS1
Protein Accession Number:
Q07955
Protein Gi Number:
730773
Ncbi Gene Id Number:
6426
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SFRS1 is a member of the arginine/serine-rich splicing factor protein family, and functions in both constitutive and alternative pre-mRNA splicing. The protein binds to pre-mRNA transcripts and components of the spliceosome, and can either activate or repress splicing depending on the location of the pre-mRNA binding site. The protein's ability to activate splicing is regulated by phosphorylation and interactions with other splicing factor associated proteins.
Background Reference 1:
Sugiyama,N., et.al., Mol. Cell Proteomics(6), 1103-1109 (2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-506

List Size:
400 ul

List Price:

$495.00

Datasheet
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