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Western blot analysis of extracts of various cell lines, using SFTPA1 antibody (18-957) at 1:200 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
Western blot analysis of extracts of various cell lines, using SFTPA1 antibody (18-957) at 1:200 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.

SFTPA1 Antibody

CATALOG NUMBER: 18-957

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
A synthetic peptide of human SFTPA1
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:500 - 1:1000
Positive Control 1:
NCI-H460
Positive Control 2:
NCI-H125
Positive Control 3:
NIH/3T3
Positive Control 4:
3T3L1
Positive Control 5:
Mouse intestine
Predicted Molecular Weight:
Observed: 35kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SFTPA1
Additional Names:
SFTPA1, surfactant, pulmonary-associated protein A1B, pulmonary-associated protein A1A, surfactant protein A1B, surfactant protein A1 variant AD' 6A4, surfactant protein A1 variant AD' 6A3, surfactant protein A1 variant AD' 6A2, surfactant protein A1 variant AD' 6A, surfactant protein A1 variant ACD' 6A4, surfactant protein A1 variant ACD' 6A3, surfactant protein A1 variant ACD' 6A2, surfactant protein A1 variant AB'D' 6A4, surfactant protein A1 variant AB'D' 6A3, surfactant protein A1 variant AB'D' 6A2, surfactant protein A1, SP-A1, SPA1, SP-A, SPA, SFTPA1B, SFTP1, pulmonary surfactant-associated protein A1, PSP-A, PSPA, PSAP, OTTHUMP00000019931, OTTHUMP00000019930, OTTHUMP00000019929, OTTHUMP00000019928, MGC198590, MGC133365, FLJ99559, FLJ79095, FLJ77898, FLJ61144, FLJ51913, FLJ50593, collectin-4, COLEC4, alveolar proteinosis protein, 35 kDa pulmonary surfactant-associated protein, SFTPA
Ncbi Gene Id Number:
653509
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a lung surfactant protein that is a member of a subfamily of C-type lectins called collectins. The encoded protein binds specific carbohydrate moieties found on lipids and on the surface of microorganisms. This protein plays an essential role in surfactant homeostasis and in the defense against respiratory pathogens. Mutations in this gene are associated with idiopathic pulmonary fibrosis. Alternate splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-957

List Size:
100 uL

List Price:

$436.00

Datasheet
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