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42-993 (0.01ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-993 (5ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-993 (0.01ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

SFTPA1 Antibody

CATALOG NUMBER: 42-993

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Cow
Immunogen:
The immunogen for this antibody is: C-HLDEELQATLHDFR
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 30kDa band observed in Human Lung lysates (calculated MW of 27.7kDa according to NP_001087239.2). Recommended concentration: 0.01-0.03ug/ml.Immunohistochemistry:Paraffin embedded Human Lung. Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognize all the reported isoforms of A1 (NP_005402.3; NP_001087239.2; NP_001158117.1; NP_001158118.1) and A2 ( NP_001092138.1), Reported variants represent identical protein: NP_001158116.1, NP_005402.3, NP_001158119.1
Positive Control 1:
Cat. No. 1331 - Human Lung Tissue Lysate
Predicted Molecular Weight:
Approx 30 kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SFTPA1
Additional Names:
SFTPA1, surfactant protein A1, COLEC4, FLJ50593, FLJ51913, FLJ61144, FLJ77898, FLJ79095, FLJ99559, MGC133365, MGC198590, PSAP, PSP-A, PSPA, SFTP1, SFTPA1B, SP-A, SP-A1, SPA, SPA1, 35 kDa pulmonary surfactant-associated protein, OTTHUMP00000019928, OTTHUMP00000019929, OTTHUMP00000019930, OTTHUMP00000019931, alveolar proteinosis protein, collectin-4, pulmonary surfactant-associated protein A1, surfactant protein A1 variant AB'D' 6A2, surfactant protein A1 variant AB'D' 6A3, surfactant protein A1 variant AB'D' 6A4, surfactant protein A1 variant ACD' 6A2, surfactant protein A1 variant ACD' 6A3, surfactant protein A1 variant ACD' 6A4, surfactant protein A1 variant AD' 6A, surfactant protein A1 variant AD' 6A2, surfactant protein A1 variant AD' 6A3, surfactant protein A1 variant AD' 6A4, surfactant protein A1B, surfactant, pulmonary-associated protein A1A, surfactant, pulmonary-associated protein A1B
Protein Accession Number:
NP_005402.3,NP_001087239.2,NP_001158117.1,NP_001158118.1,NP_001092138.1
Protein Gi Number:
38888175
Ncbi Gene Id Number:
653509;729238

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-993

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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