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Western blot analysis of SGF29 Antibody in MDA-MB435 cell line lysates (35ug/lane)
SGF29 Antibody IHC analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of SGF29 Antibody in MDA-MB435 cell line lysates (35ug/lane)

SGF29 Antibody

CATALOG NUMBER: 61-809

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Chicken, Mouse, Rat
Immunogen:
This SGF29 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 179-208 amino acids from the C-terminal region of human SGF29.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
33 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CCDC101
Additional Names:
SAGA-associated factor 29 homolog, Coiled-coil domain-containing protein 101, CCDC101, SGF29
Protein Accession Number:
Q96ES7
Protein Gi Number:
74731608
Ncbi Gene Id Number:
112869
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CCDC101 is a subunit of 2 histone acetyltransferase complexes: the ADA2A (TADA2A; MIM 602276)-containing (ATAC) complex and the SPT3 (SUPT3H; MIM 602947)-TAF9 (MIM 600822)-GCN5 (KAT2A; MIM 602301)/PCAF (KAT2B; MIM 602303) acetylase (STAGA) complex. Both of these complexes contain either GCN5 or PCAF, which are paralogous acetyltransferases.
Background Reference 1:
Imielinski, M., et al. Nat. Genet. 41(12):1335-1340(2009)
Background Reference 2:
Wang, Y.L., et al. J. Biol. Chem. 283(49):33808-33815(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-809

List Size:
400 ul

List Price:

$495.00

Datasheet
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