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Western blot analysis of SGK1 in human stomach tissue lysate with SGK1 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of SGK1 in human stomach tissue with SGK1 antibody at 5 μg/mL.
Immunofluorescence of SGK1 in human stomach tissue with SGK1 antibody at 20 μg/mL.
Western blot analysis of SGK1 in human stomach tissue lysate with SGK1 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

SGK1 Antibody

CATALOG NUMBER: 6319

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (89%), Chicken: (84%), Rabbit: (89%)
Immunogen:
SGK1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SGK1.
The immunogen is located within amino acids 370 - 420 of SGK1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
SGK1 antibody can be used for detection of SGK1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
SGK1 antibody is predicted to not cross-react with other SGK protein family members. At least five isoforms are known to exist
Positive Control 1:
Cat. No. 1319 - Human Stomach Tissue Lysate
Positive Control 2:
Cat. No. 10-801 - Human Stomach Tissue Slide
Purification:
SGK1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SGK1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SGK1 antibody can be stored at 4 °C for 3 months and -20 °C stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SGK1
Additional Names:
SGK1 Antibody: SGK, SGK
Protein Accession Number:
NP_005618
Protein Gi Number:
25168263
Ncbi Gene Id Number:
6446
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SGK1 Antibody: Serum and glucocorticoid-regulated protein kinase 1 (SGK1) is a member of the AGC family of serine/threonine kinases that plays an important role in cellular stress response and cell survival. SGK1 is transcriptionally activated by numerous stimuli, including serum, glucocorticoids, osmotic stress, and hormones. SGK1 functions as a regulator of cell survival and ion channels and transporters such as ENaC or KCNA3/Kv1.3. It phosphorylates and negatively regulates pro-apoptotic FOXO3A. SGK1 is thought to play an essential function in nephropathy and cardiovascular development.
Background Reference 1:
Webster MK, Goya L, Ge Y, et al. Characterization of sgk, a novel member of the serine/threonine protein kinase gene family which is transcriptionally induced by glucocorticoids and serum. Mol. Cell Biol. 1993; 13:2031-40.
Background Reference 2:
Leong ML, Maiyar AC, Kim B, et al. Expression of the serum- and glucocorticoid-inducible protein kinase, Sgk, is a cell survival response to multiple types of environmental stress stimuli in mammary epithelial cells. J. Biol. Chem. 2003; 278:5871-82.
Background Reference 3:
Firestone GL, Giampaolo JR, O'Keeffe BA, et al. Stimulus-dependent regulation of serum and glucocorticoid inducible protein kinase (SGK) transcription, subcellular localization and enzymatic activity. Cell. Physiol. Biochem. 2003; 13:1-12.
Background Reference 4:
Brunet A, Park J, Tran H, et al. Protein kinase SGK mediates survival signals by phosphorylating the forkhead transcription factor FKHRL1 (FOXO3a). Mol. Cell Biol. 2001; 21:952-65.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6319

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

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