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Western blot analysis in MCF-7,CEM,NCI-H460 cell line lysates (35ug/lane).
SGMS2 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in MCF-7,CEM,NCI-H460 cell line lysates (35ug/lane).

SGMS2 Antibody

CATALOG NUMBER: 64-125

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Monkey, Mouse, Rat
Immunogen:
This SGMS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 338-365 amino acids from the C-terminal region of human SGMS2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
42 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SGMS2
Additional Names:
Phosphatidylcholine:ceramide cholinephosphotransferase 2, Sphingomyelin synthase 2, SGMS2, SMS2
Protein Accession Number:
Q8NHU3
Protein Gi Number:
44888519
Ncbi Gene Id Number:
166929
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Sphingomyelin, a major component of cell and Golgi membranes, is made by the transfer of phosphocholine from phosphatidylcholine onto ceramide, with diacylglycerol as a side product. The protein encoded by this gene is an enzyme that catalyzes this reaction primarily at the cell membrane. The synthesis is reversible, and this enzyme can catalyze the reaction in either direction. The encoded protein is required for cell growth. Three transcript variants encoding the same protein have been found for this gene.
Background Reference 1:
Ternes, P., et al. J. Lipid Res. 50(11):2270-2277(2009) Liu, J., et al. Arterioscler. Thromb. Vasc. Biol. 29(6):850-856(2009) Tani, M., et al. Biochem. Biophys. Res. Commun. 381(3):328-332(2009) Wang, W., et al. Plant Cell 20(11):3163-3179(2008) Yeang, C., et al. Biochim. Biophys. Acta 1781(10):610-617(2008) Villani, M., et al. Biochem. J. 414(1):31-41(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

64-125

List Size:
400 ul

List Price:

$469.00

Out of stock

Datasheet
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