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Western blot analysis in MCF-7 cell line lysates (35ug/lane).
Western blot analysis in MCF-7 cell line lysates (35ug/lane).

SGOL1 Antibody

CATALOG NUMBER: 59-021

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This SGOL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 339-367 amino acids from the Central region of human SGOL1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
64 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SGOL1
Additional Names:
Shugoshin-like 1, hSgo1, Serologically defined breast cancer antigen NY-BR-85, SGOL1, SGO1
Protein Accession Number:
Q5FBB7
Protein Gi Number:
74741474
Ncbi Gene Id Number:
151648
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SGOL1 plays a central role in chromosome cohesion during mitosis by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. May act by preventing phosphorylation of the STAG2 subunit of cohesin complex at the centromere, ensuring cohesin persistence at centromere until cohesin cleavage by ESPL1/separase at anaphase. Essential for proper chromosome segregation during mitosis and this function requires interaction with PPP2R1A. Its phosphorylated form is necessary for chromosome congression and for the proper attachment of spindle microtubule to the kinetochore. Necessary for kinetochore localization of PLK1 and CENPF. May play a role in the tension sensing mechanism of the spindle-assembly checkpoint by regulating PLK1 kinetochore affinity. Isoform 3 plays a role in maintaining centriole cohesion involved in controlling spindle pole integrity.
Background Reference 1:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 2:
Okamoto, N., et al. Genes Cells 15(5):471-484(2010)
Background Reference 3:
Xu, Z., et al. Mol. Cell 35(4):426-441(2009)
Background Reference 4:
Gambe, A.E., et al. FEBS Lett. 583(12):1951-1956(2009)

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

59-021

List Size:
400 ul

List Price:

$495.00

Datasheet
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