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Western blot analysis of SGSM3 in 293 cell lysate with SGSM3 antibody at (A) 1 and (B) 2 μg/mL.
Immunofluorescence of SGSM3 in 293 cells with SGSM3 antibody at 20 μg/mL.
Western blot analysis of SGSM3 in 293 cell lysate with SGSM3 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

SGSM3 Antibody

CATALOG NUMBER: 7037

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (82%), Mouse: (82%), Bovine: (79%)
Immunogen:
SGSM3 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human SGSM3.
The immunogen is located within amino acids 30 - 80 of SGSM3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
SGSM3 antibody can be used for detection of SGSM3 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
Multiple isoforms of SGSM3 are known to exist. SGSM3 antibody is predicted to not cross react with other SGSM family proteins.
Positive Control 1:
Cat. No. 1210 - HEK293 Cell Lysate
Positive Control 2:
Cat. No. 17-010 - HEK293 Cell Slide
Predicted Molecular Weight:
82 kDa
Purification:
SGSM3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SGSM3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SGSM3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SGSM3
Additional Names:
SGSM3 Antibody: MAP, RUSC3, RUTBC3, RABGAP5, RabGAP-5, MAP, RABGAPLP, Small G protein signaling modulator 3, Merlin-associated protein, RabGAPLP
Protein Accession Number:
CAQ08933
Protein Gi Number:
168985143
Ncbi Gene Id Number:
27352
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SGSM3 Antibody: Small G proteins such as RAP and RAB proteins are the key molecules in intracellular signal transduction and vesicle transportation.  A novel protein family small G protein signaling modulator (SGSM) consisting of three members SGSM1-3 bind to RAP and RAB family proteins. All three SGSM proteins possess both a RUN domain and a TBC domain. Little is known of the exact function of SGSM3.
Background Reference 1:
Bar-Sagi D and Hall A. Ras and Rho GTPases: a family reunion. Cell 2000; 103:227-38.
Background Reference 2:
Colicelli J. Human ras superfamily proteins and related GTPases. Sci. STKE 2004; 250:RE13.
Background Reference 3:
Yang H, Sasaki T, Minoshima S, et al. Identification of three novel proteins (SGSM1, 2, 3) which modulate small G protein (RAP and RAB)-mediated signaling pathway. Genomics 2007; 90:249-60.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7037

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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