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46-365 staining (0.3ug/ml) of Human Thymus lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
HEK293 overexpressing SH2D1A and probed with 46-365 (mock transfection in first lane).
46-365 staining (0.3ug/ml) of Human Thymus lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

SH2D1A Antibody

CATALOG NUMBER: 46-365

Clonality:
Polyclonal
Tested Applications:
ELISA, IP, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-HKRYFRKIKN
Conjugate:
Unconjugated
Tested Applications:
ELISA, IP, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 16kDa band observed in Human Thymus lysates (calculated MW of 14.2xkDa according to NP_002342.1). In transfected HEK293 transiently expressing SH2D1A a band of approx. 16kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1ug/ml.Immunoprecipitation:This antibody was deemed fit for IP under native conditions (observations from anonymous customer).
Positive Control 1:
Cat. No. 1314 - Human Thymus Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SH2D1A
Additional Names:
SH2D1A, SAP, SH2 domain protein 1A, Duncan's disease (lymphoproliferative syndrome), HGNC:10820, DSHP, EBVS, IMD5, LYP, MTCP1, XLP, XLPD , SH2 domain protein 1A, SLAM (signaling lymphocyte activation molecule) associated protein, RP5-1052M9.3, FLJ18687, FLJ92177, SLAM-associated protein, T cell signal transduction molecule SAP, signaling lymphocyte activation molecule-associated protein
Protein Accession Number:
NP_002342.1,NP_001108409.1
Protein Gi Number:
4506923
Ncbi Gene Id Number:
4068
Background Reference 1:
Mikhalap SV, Shlapatska LM, Yurchenko OV, Yurchenko MY, Berdova GG, Nichols KE, Clark EA, Sidorenko SP. The adaptor protein SH2D1A regulates signaling through CD150 (SLAM) in B cells. Blood. 2004 Dec 15;104(13):4063-70. Epub 2004 Aug 17.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-365

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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