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Western blot analysis in NCI-H292 cell line lysates (35ug/lane).
SH2D4A Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in NCI-H292 cell line lysates (35ug/lane).

SH2D4A Antibody

CATALOG NUMBER: 56-202

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This SH2D4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 38-67 amino acids from the N-terminal region of human SH2D4A.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
53 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SH2D4A
Additional Names:
SH2 domain-containing protein 4A, Protein SH(2)A, Protein phosphatase 1 regulatory subunit 38, SH2D4A, PPP1R38, SH2A
Protein Accession Number:
Q9H788
Protein Gi Number:
74725117
Ncbi Gene Id Number:
63898
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SH2D4A inhibits estrogen-induced cell proliferation by competing with PLCG for binding to ESR1, blocking the effect of estrogen on PLCG and repressing estrogen-induced proliferation. May play a role in T-cell development and function.
Background Reference 1:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 2:
Li, T., et al. BMB Rep 42(8):516-522(2009)
Background Reference 3:
Venkatesan, K., et al. Nat. Methods 6(1):83-90(2009)
Background Reference 4:
Lapinski, P.E., et al. J. Immunol. 181(3):2019-2027(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-202

List Size:
400 ul

List Price:

$495.00

Datasheet
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