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Western blot analysis of SH3BP4 in rat lung tissue lysate with SH3BP4 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of SH3BP4 in human lung tissue with SH3BP4 antibody at 5 μg/mL.
Western blot analysis of SH3BP4 in rat lung tissue lysate with SH3BP4 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

SH3BP4 Antibody

CATALOG NUMBER: 5167

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
SH3BP4 antibody was raised against an 18 amino acid synthetic peptide from near the carboxy terminus of human SH3BP4.
The immunogen is located within amino acids 860 - 910 of SH3BP4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
SH3BP4 antibody can be used for detection of SH3BP4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in rat samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1462 - Rat Lung Tissue Lysate
Positive Control 2:
Cat. No. 10-101 - Human Lung Tissue Slide
Purification:
SH3BP4 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SH3BP4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SH3BP4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SH3BP4
Additional Names:
SH3BP4 Antibody: TTP, BOG25, EHB10, TTP, SH3 domain-binding protein 4, EH-binding protein 10
Protein Accession Number:
NP_055336
Protein Gi Number:
7657562
Ncbi Gene Id Number:
23677
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SH3BP4 Antibody: SH3BP4 was initially isolated as a novel cDNA using differential display analysis using cultured human corneal fibroblasts. The protein contains three Asn-Pro-Phe (NPF) motifs, an SH3 domain, a PXXP motif, a bipartite nuclear targeting signal, and a tyrosine phosphorylation site. SH3BP4 has been shown to interact with specific endocytic proteins such as clathrin, dynamin, and the transferrin receptor (TfR) and localizes to TfR-containing coated pits and vesicles. It is thought to be involved in cargo-specific control of clathrin-mediated endocytosis, specifically controlling the internalization of TfR. At least two isoforms of SH3BP4 are known to exist.
Background Reference 1:
Dunlevy JR, Berryhill BL, Vergnes JP, et al. Cloning, chromosomal localization, and characterization of cDNA from a novel gene, SH3BP4, expressed by human corneal fibroblasts. Genomics1999; 62:519-24.
Background Reference 2:
Tosoni D, Puri C, Confalonieri S, et al. TTP specifically regulates the internalization of the transferrin receptor. Cell2005; 123:875-888.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5167

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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