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Western blot analysis of extracts of various cell lines, using SH3GL3 antibody (19-283) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Western blot analysis of extracts of various cell lines, using SH3GL3 antibody (19-283) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

SH3GL3 Antibody

CATALOG NUMBER: 19-283

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human SH3GL3 (NP_003018.3).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:4000
IHC: 1:100 - 1:200
Positive Control 1:
Mouse brain
Positive Control 2:
Mouse liver
Positive Control 3:
Rat testis
Positive Control 4:
Rat brain
Predicted Molecular Weight:
Observed: 39kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SH3GL3
Additional Names:
CNSA3, EEN-B2, HsT19371, SH3D2C, SH3P13, endophilin-A3, SH3 domain containing GRB2 like endophilin A3, SH3 domain protein 2C, SH3 domain-containing GRB2-like protein 3, SH3-domain GRB2-like 3, endophilin-3
Ncbi Gene Id Number:
6457
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-283

List Size:
100 uL

List Price:

$436.00

Datasheet
Shipping Info

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Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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