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Western blot analysis of SH3RF2 in 3T3 cell lysate with SH3RF2 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of SH3RF2 in human brain tissue with SH3RF2 antibody at 10 μg/mL.
Immunofluorescence of SH3RF2 in human brain tissue with SH3RF2 antibody at 20 μg/mL.
Western blot analysis of SH3RF2 in 3T3 cell lysate with SH3RF2 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

SH3RF2 Antibody

CATALOG NUMBER: 5887

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
SH3RF2 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human SH3RF2.
The immunogen is located within the last 50 amino acids of SH3RF2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
SH3RF2 antibody can be used for detection of SH3RF2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
SH3RF2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SH3RF2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SH3RF2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SH3RF2
Additional Names:
SH3RF2 Antibody: HEPP1, POSHER, RNF158, PPP1R39, Putative E3 ubiquitin-protein ligase SH3RF2, Heart protein phosphatase 1-binding protein, HEPP1
Protein Accession Number:
NP_689763
Protein Gi Number:
222446607
Ncbi Gene Id Number:
153769
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SH3RF2 Antibody: Although the SH3 domain containing ring finger 2 protein (SH3RF2) has been identified as a marker of the nucleus accumbens in the human brain, little is known about this protein. Recent yeast two-hybrid experiments have shown that SH3RF2 associates with protein phosphatase 1 (PP1) and is expressed primarily in heart and testis tissue. Recombinant SH3RF2 enhanced PP1 enzymatic activity and antagonized the ability of phopho-inhibitor-1 or -2 to inhibit PP1, suggesting that SH3RF2 may play a role working with PP1 in cardiac functions.
Background Reference 1:
Ernst C, Sequeira A, Klempan, et al. Confirmation of region-specific patterns of gene expression in the human brain. Neurogenetics2007; 8:219-24.
Background Reference 2:
Chen CY, Lai NS, Yang JJ, et al. FLJ23654 encodes a heart protein phosphatase 1-binding protein (Hepp1). Biochem. Biophys. Res. Commun.2010; 391:698-702.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5887

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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